ABSTRACT
This study was undertaken to examine the effects of ultraviolet light (UVL) and rebamipide on the cutaneous blood flow and tissue survival on rabbit skin flap. In a random bipedicle flap, Laser Doppler Flowmetry (LDF) was employed to measure the blood flow of flap (BFF). Wound Margin Strength (WMS) measured by force transducer and Light microscopy were used for evaluation of tissue viability. Single exposure to UVL increased the BFF gradually for more than 15 hours, and decreased the vasoconstrictor effect of intravenous phenylephrine. The UVL-induced increase in BFF regressed after 18 hours of irradiation, and this regression was tended to be enhanced by intradermal injection of L-NAME, a nitric oxide synthase (NOS) inhibitor, but the regression was significantly reversed by acetylcholine, an endothelial constitutive NOS (cNOS) activator and L-arginine, an NO precursor. Rebamipide, a novel antiulcer oxide synthase (NOS) inhibitor, but the regression was significantly reversed by acetylcholine, an endoagent known to scavenge the hydroxyl radical, abruptly reversed the spontaneous regression of the UVL-induced increase in BFF by the same manner as L-arginine. In ischemic skin flap, rebamipide increased the BFF abruptly by the same manner as sodium nitroprusside (SNP), an NO doner, while N-acetylcystein (NAC), a free radical scavenger, gradually increase the BFF. The rebamipide-induced increase in BFF was sustained at the level of the SNP-induced increase in BFF during the late period of experiment. Rebamipide increased the WMS of skin flaps and prevented the tissue necrosis in comparison with L-NAME. Based on these results, it is concluded that in rabbit skin, UVL irradiation increases the BFF by NO release, and rebamipide exerts a protective effect on the viability of ischemic skin flaps by either or both the increase in BFF by NO release and free radical scavenger effect.
Subject(s)
Acetylcholine , Arginine , Hydroxyl Radical , Injections, Intradermal , Laser-Doppler Flowmetry , Microscopy , Necrosis , NG-Nitroarginine Methyl Ester , Nitric Oxide , Nitric Oxide Synthase , Nitroprusside , Phenylephrine , Skin , Tissue Survival , Transducers , Ultraviolet Rays , Wounds and InjuriesABSTRACT
Alopecia areata(AA) is a common dermatologic disease, the origin of which is still unknown. At least, it has been associated with some genetic influences in the etiopathogenesis. We have now evaluated AA patients to study the actual association of the genetic role by fingerprint analysis. The incidences of six or more arches were determined in 74 female and 86 male patients excluding alopecia totalis and universalis, and in healthy 167 female controls, based on Selmanowitz's thesis (1974). The results of this study are follows: 1. The difference between the frequency in AA patients(7. 5%) and in controls (3.3%) reaches the statistical significance(p<0.05 by x2-test). 2. The association of genetic background in AA is strengthened in the group of female patients(p<0. 01), recurrent episode(p<0.01). and multiplex farm (p<0.01). 3. Twenty patients(12. 5%) have family history. The incidence in 3(15.0%) of these patients is considerably common but not statistically significant. A correlation of biologic relevance and family predisposition in AA is not precluded. 4. The frequency of arches in patients and in controls is increased on both second fingers and decreased on both fourth fingers. It is more common on both second and left third fingers in female patients. The incidence of arches averages about 6 67% in patients and 4. 58% in controls.
Subject(s)
Female , Humans , Male , Alopecia Areata , Alopecia , Dermatoglyphics , Fingers , IncidenceABSTRACT
We report herein a case of polymorphic pemphigoid in a 65-year-old woman, who had one-year history of generlized polymorphic eruption with intensely pruritic excoriated vesicobiullous lesions and residual pigmentation. Face and oral mucosa were spared. The skin biopsy specimen showed a subepidermal blister containing many eosinophils. Linear deposition of 1gG and C3 along the basement membrane was noticed on direct irnmunofluorescerice. Polyclonal garnmopathy with a tendency of beta-gamma bridging and increased IgG, IgA and kappachain, was frund on serum protein electrophoresis and immunoelectrophoresis. Skin lesions were well coritrolled by a combined therapy of prednisolone(20 mg/d), dapsone(100 mg/d) and cyclophosphamide(100 mg/d) for 4 months. Direct immunofluorescence performed 11 rnonths after the cnmpletion of the therapy was negative.
Subject(s)
Aged , Female , Humans , Basement Membrane , Biopsy , Blister , Electrophoresis , Eosinophils , Fluorescent Antibody Technique, Direct , Immunoelectrophoresis , Immunoglobulin A , Immunoglobulin G , Mouth Mucosa , Pemphigoid, Bullous , Pigmentation , SkinABSTRACT
We experienced a case of granulation tissue occuring in lateral sulci of the right thumb, index, middle and left thumb, middle finger nails in 71-year-old psoriatic male patient taking 5pmg daily(0.6mg/kg/day) during 4th week of etretinate therapy. The excision of the granulation lesion and partial nail ablation was performed and his doses was lowered to 25mg daily (0. 3mg/kg/day). The condition were resolved with no recurrence 4 weeks later after the operation.
Subject(s)
Aged , Humans , Male , Acitretin , Etretinate , Fingers , Granulation Tissue , Nails , Recurrence , ThumbABSTRACT
We report here-in a case of benign hemangioendothelioma occurring in a 2 day-old male neonate on central portion of lumbar area. The lesion was movable, and elevated firm nodule, measuring 1.5x2.0 cm in size. For the purpose of diagnosis and therapy, excisional biopsy was performed, which revealed typical histopathologic pictures in the deep dermis and subcutaneous area. The lesion showed no recurrence at follow-up check after 6 months.